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In vivo evaluation of analgesic and antipyretic activities of piceatannol-richextract from Senna garrettiana heartwood |
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| รหัสดีโอไอ | |
| Creator | 1. Suparada Surapanthanakorn 2. Narubodee Phadoongsombut 3. Chatchai Wattanapiromsakul 4. Wantana Reanmongkol |
| Title | In vivo evaluation of analgesic and antipyretic activities of piceatannol-richextract from Senna garrettiana heartwood |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2560 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 39 |
| Journal No. | 5 |
| Page no. | 589 |
| Keyword | S. garrettiana extract,piceatannol-rich,pain,fever,animal models |
| ISSN | 0125-3395 |
| Abstract | A methanolic extract from Senna garrettiana (S. garrettiana) heartwood was prepared and then a fractionation processwas performed to obtain hexane, dichloromethane, ethyl acetate, and aqueous fractions. An antinociceptive screening of eachfraction was carried out using the acetic acid-induced writhing in mice. Among all the fractions, the ethyl acetate fractionshowed the highest activity on the writhing test. The ethyl acetate fraction was separated to obtain a piceatannol-richextract. The S. garrettiana extract contains 11.70 % w/w and 39.16 % w/w piceatannol in the ethyl acetate fraction and thepiceatannol-rich extract, respectively. The analgesic activities of the ethyl acetate fraction (50, 100 and 200 mg/kg) and thepiceatannol-rich extract (10, 20 and 40 mg/kg) were evaluated by the acetic acid-induced writhing test, hot-plate test andformalin test. The antipyretic activity of these extracts was assessed on yeast's-induced pyrexia in rats. The acute toxicitywas also investigated. In the acute toxicity study, no lethality was observed after the oral administration of methanolic extractof S. garrettiana heartwood even at a high dose of 5 g/kg in mice. The oral administration of the ethyl acetate fractiondecreased the number of writhings in a dose dependent manner with 54.9 %, 68.5 %, and 71.0 % inhibition, respectively.A similar result was also observed after the oral administration of the piceatannol-rich extract with 53.1%, 69.2% and 80.3%inhibition, respectively. In the formalin test, either the ethyl acetate fraction or the piceatannol-rich extract significantlydiminished the licking time in both the early and late phases. Neither the ethyl acetate nor the piceatannol-rich extract hadany effect on heat-induced pain. The ethyl acetate fraction at the same dosage range significantly decreased the rat rectaltemperature at 2, 3 and 4 hrs. The piceatannol-rich extract at a dose of 20 and 40 mg/kg suppressed the rectal temperature overthe same time intervals. These results demonstrated that the ethyl acetate fraction and the piceatannol-rich extract fromS. garrettiana heartwood possessed analgesic and antipyretic activities with an apparently similar efficacy. The probablemechanism(s) of analgesic actions might be mediated by both the peripheral and central mechanisms |
