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RAPD fingerprinting and genetic relationship of Gardenia species in Thailand |
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| รหัสดีโอไอ | |
| Creator | 1. Onuma Zongram 2. Nijsiri Ruangrungsi 3. Kanchana Rungsihirunrat |
| Title | RAPD fingerprinting and genetic relationship of Gardenia species in Thailand |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2560 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 39 |
| Journal No. | 4 |
| Page no. | 471 |
| Keyword | Gardenia,genetic relationship,RAPD analysis,DNA fingerprint |
| ISSN | 0125-3395 |
| Abstract | DNA based molecular markers have a potential utility in herbal medicine analysis and widely used for studying geneticrelationship of medicinal plant species. Therefore, this study aims to assess the genetic relationship among eleven Gardeniaspecies collected from different locations in Thailand using random amplified polymorphic DNA (RAPD) marker. Ninetyprimers were initially screened, out of which 20 primers generated 579 reproducible bands of different sizes with an averageof 28.95 bands per primer. The mean percentage of polymorphic bands was 99.5%. Similarity index ranged from 0.089 to0.332. The highest similarity index (0.332) was found between Gardenia lineata and G. jasminoides while the lowestsimilarity index (0.089) was found between G. carinata and G. sootepensis. A dendrogram was constructed using theunweighted pair-group method with arithmetic averages (UPGMA) and can be divided into 2 distinct clusters whichcorrelated with their morphological characteristics. |
