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Comparative analysis of fliC Gene from Salmonella enterica sub-speciesfor biosensor probe design and phylogenetic tree construction |
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| รหัสดีโอไอ | |
| Creator | 1. Sunderamurthe Satisvar 2. Subash Chandra Bose Gopinath 3. Mohd Khairuddin Md Arshad 4. Dee Vie Ong 5. Thangavel Lakshmipriya 6. Chun Hong Voon 7. Uda Hashim 8. Abdul Rahim Ruslinda 9. Suresh Venkata Chinni |
| Title | Comparative analysis of fliC Gene from Salmonella enterica sub-speciesfor biosensor probe design and phylogenetic tree construction |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2560 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 39 |
| Journal No. | 3 |
| Page no. | 373 |
| Keyword | Salmonella enterica,sub-species,fliC gene,flagellin,H antigen,biosensor |
| ISSN | 0125-3395 |
| Abstract | In tandem to the Salmonellosis infection worldwide, a study was conducted to determine the conserved and nonconservedregion in Salmonella enterica sub-species so that it can be used to design probes in biosensors for the detectionof Salmonella enterica as a species or sub-species distinctively. The region was selected for this study through fliC genethat is present in all Salmonella sub-species, encodes the Salmonella flagella or flagellin determines the serotype due toits H antigen and provides virulence to the bacteria. All sub-species were analyzed in a group of six and then analyzedindividually in groups of two with all possible combinations to determine the overlapping regions. Based on the study, thepredominant conserved region for S. enterica sub-species is between 103 and 158, whereas the non-conserved region is from1245 to 1285. A phylogenetic tree was constructed for the S. enterica sub-species to determine the evolution of theSalmonella sub-species. |
