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Occurrence and effectiveness of indigenous Metarhizium anisopliaeagainst adults Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae)in Southern Thailand |
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รหัสดีโอไอ | |
Creator | 1. Narit Thaochan 2. Wiwat Sausa-Ard |
Title | Occurrence and effectiveness of indigenous Metarhizium anisopliaeagainst adults Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae)in Southern Thailand |
Publisher | Research and Development Office, Prince of Songkla University |
Publication Year | 2560 |
Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
Journal Vol. | 39 |
Journal No. | 3 |
Page no. | 325 |
Keyword | indigenous entomopathogenic fungi,Metarhizium anisopliae,biocontrol agents,Zeugodacus cucurbitae |
ISSN | 0125-3395 |
Abstract | The indigenous entomopathogenic fungus, Metarhizium anisopliae, was isolated from soil samples and infectedinsect cadavers in fruit orchards of peninsular Thailand. Sixty-four isolates of indigenous M. anisopliae were obtained fromfruit orchards in Nakhon Si Thammarat, Patthalung, and Songkhla provinces by using selective media. The soil samples fromfruit orchards of Songkhla province gave the most fungal isolates (39), while the soil samples from Patthalung and Nakhon SiThammarat gave 14 and 5 isolates, respectively. Six more isolates were from the infected insect cadavers, collected from theorchards in Nakhon Si Thammarat (4) and Songkhla (2). The conidial morphologies of the isolates were divided into fourgroups based on length to width (L/W) ratio: 1.69-1.96, 2.01-2.47, 2.55-2.94, and 3.14-3.16. The efficiency of isolatedM. anisopliae against adult stage Zeugodacus cucurbitae was evaluated in laboratory conditions. The fungal isolatesPSUM02, PSUM05 and PSUM08 were the most effective with complete 100% mortality of Z. cucurbitae within seven daysfrom inoculation. These fungi in the soils of different fruit orchards should be further assessed for their efficacies againstdifferent insect pests. In particular, future studies should look into the three efficient isolates identified, and determine theirkey differences from the other isolates representing the same habitat or geological origin. |