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Biological activity of Penaeus monodon GILT in shrimp pathogen protection |
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| รหัสดีโอไอ | |
| Creator | 1. Aekkaraj Nualla-ong 2. Hirun Saelim 3. Kittima Kongton 4. Amornrat Phongdara |
| Title | Biological activity of Penaeus monodon GILT in shrimp pathogen protection |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2560 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 39 |
| Journal No. | 2 |
| Page no. | 261 |
| Keyword | shrimp (Penaeus monodon),gamma-interferon-inducible lysosomal thiol reductase (GILT),enzymatic activity,neutralization,antimicrobial assay |
| ISSN | 0125-3395 |
| Abstract | Gamma-interferon-inducible lysosomal thiol reductase (GILT) contains a CXXC active site motif that possesses thiolreductase activity by catalyzing the disulfide bond reduction of exogenous antigens. Mutating the active site of human GILTto change the cysteine residues to serine residues eliminates this property. Our previous study reported that Penaeusmonodon GILT (PmGILT) contained a CXXS active site motif. Therefore, we assessed the enzymatic activity of PmGILT anddemonstrated that it displayed identical thiol reductase activity at an acidic pH. In addition, the biological activity of PmGILTagainst shrimp pathogens, including white spot syndrome virus (WSSV) and Gram-negative bacteria, was investigated.The neutralization of WSSV with PmGILT indicated the inhibition of WSSV invasion into shrimp hemocyte cells. Moreover,the relative percentage survival of shrimp injected with PmGILT-treated virus solution was 75%. Finally, the antimicrobialactivity of PmGILT was confirmed by the growth inhibition of Vibrio harveyi. These results establish the role of PmGILT inthe inhibition of the virulence of two major shrimp pathogens. |
