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An on-line LC-MS/ DPPH approach towards the quality control ofantioxidative ingredients in Sahastara |
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| รหัสดีโอไอ | |
| Creator | 1. Nitra Nuengchamnong 2. Kornkanok Ingkaninan |
| Title | An on-line LC-MS/ DPPH approach towards the quality control ofantioxidative ingredients in Sahastara |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2560 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 39 |
| Journal No. | 1 |
| Page no. | 123 |
| Keyword | DPPH assay,holistic,LC-MS,on-line chromatography,herbal medicine |
| ISSN | 0125-3395 |
| Abstract | The holistic approach of herbal medicines is becoming acceptable in many parts of the world. In order to determine,not only chemical but also biological activity profiles of traditional medicines, on-line liquid chromatography-electrosprayionization-tandem mass spectrometry (LC-ESI-MS/MS) coupled to antioxidant assay was introduced. Here, we demonstratethe performance of this on-line monitor for quantitatively and qualitatively analyses of antioxidants in Sahasthara, a Thaitraditional medicine. HPLC real-time coupling to ESI-MS and DPPH assay was applied to separate and identify theconstituent compounds responsible for antioxidant activity. The antioxidant fingerprint was obtained together with the LC-MSchromatogram. The full scan MS and the multiple reaction monitoring (MRM) were used for structural confirmation andquantitation. Peak identification was performed by comparing retention times and mass spectra with reference compoundsand published data. Based on their mass spectra, 13 antioxidant compounds were identified as gallic acid, chebulic acid,digalloyl glucoside, caffeoylferuloylquinic lactone, galloylshikimic acid, trigalloyl glucoside, corilagin, chebulanin, chebulagicacid, dicaffeoylquinic lactone, dicaffeoyl quinic acid, ellagic acid rhamnoside and ellagic acid. Gallic acid and ellagic acidwere further quantitated and comprised 126.8?6.0 mg/g and 253.7?17.6 mg/g of Sahasthara, respectively. This on-linemonitor is shown to be an effective tool for fast and simultaneous analyses of antioxidant compounds in complex formulationscharacteristic of many traditional medicines. |
