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Reduction of the sulfoxide in glucoraphanin and sulforaphane byE. coli VL11 and BL21 (DE3) |
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| รหัสดีโอไอ | |
| Creator | 1. Vijitra Luang-In 2. Jaroslava Bob?l'ov? |
| Title | Reduction of the sulfoxide in glucoraphanin and sulforaphane byE. coli VL11 and BL21 (DE3) |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2559 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 38 |
| Journal No. | 6 |
| Page no. | 675-681 |
| Keyword | E. coli,glucosinolate,isothiocyanate,nitrile,sulfoxide reductase |
| ISSN | 0125-3395 |
| Abstract | Sulfoxide reductase activity of two Escherichia coli strains VL11 from human feces and BL21(DE3) from a molecularcloning host was expressed upon glucoraphanin induction for 16 hrs at 37?C under aerobic conditions. Bacterial inducedcultures were able to convert the sulfoxide in glucoraphanin to the sulfide and thus produced glucoerucin. Only E. coli VL11produced 30 ?M erucin and 51 ?M erucin nitrile as degradation products from 1 mM glucoraphanin metabolism whereasBL21(DE3) only biotransformed glucoraphanin to glucoerucin with 52% conversion without metabolizing it. Cell-free extractsof each E. coli strain from glucoraphnin-induced cells in citrate phosphate buffer pH 7.0 were able to convert the sulfoxidesin both glucoraphanin and sulforaphane to the sulfides and thus produced glucoerucin and erucin, respectively at 4 h underthe same incubation conditions. Sulfoxide reductases from two E. coli strains required Mg2+ ions and NADPH reducingreagents for the activity. |
