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Optimal condition to remove mercury in yellowfin tuna protein isolates andACE-inhibitory property of peptide prepared using commercial proteases |
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| รหัสดีโอไอ | |
| Creator | 1. Hathaigan Kokkaew 2. Supawan Thawornchinsombut 3. Jae Won Park |
| Title | Optimal condition to remove mercury in yellowfin tuna protein isolates andACE-inhibitory property of peptide prepared using commercial proteases |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2559 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 38 |
| Journal No. | 4 |
| Page no. | 439-447 |
| Keyword | response surface methodology,commercial enzymes,pro-drug type peptides,gastrointestinal digestion |
| ISSN | 0125-3395 |
| Abstract | Response surface methodology (RSM) was performed to maximize the mercury (Hg) reduction from yellowfin tuna(Thunnus albacare) by products protein isolates (YBPI). The optimal condition of Hg reduction (89.3%) was 10.5 mM CaCl2and a Water:YB of 12.9:1, while other variables were fixed at 5 mM citric acid, 60 min incubation, pH 11 and 8,000 x g for 15min of centrifugation. At these conditions, the significant protein recovery (80.1%) was obtained. Hydrolysates sequentiallyhydrolyzed with G6 followed by GN exhibited the highest angiotensin I-converting enzyme (ACE) inhibitory activity thanother enzyme preparations. Fractionated yellowfin tuna by products protein hydrolysate (YBPH) increased in ACE-inhibitoryactivity when peptide size decreased. In-vitro gastrointestinal (GI) digestion significantly increased bioactive property. ACEinhibitoryactivity of YBPH with and without simulated GI digestion significantly increased after incubating against ACE,demonstrating pro-drug type peptides. |
