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Investigation of antioxidative, antityrosinase and cytotoxic effectsof extract of irradiated oyster mushroom |
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| รหัสดีโอไอ | |
| Creator | 1. Nutsuda Banlangsawan 2. Bungorn Sripanidkulchai 3. Niwat Sanoamuang |
| Title | Investigation of antioxidative, antityrosinase and cytotoxic effectsof extract of irradiated oyster mushroom |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2559 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 38 |
| Journal No. | 1 |
| Page no. | 31-39 |
| Keyword | oyster mushroom (Pleurotus ostreatus),vitamin D2,antioxidant effect,cytotoxic effect,tyrosinase inhibition activity |
| ISSN | 0125-3395 |
| Abstract | Oyster mushroom (Pleurotus ostreatus Fries.) is rich in nutrition and has many medicinal properties such as antioxidantand anticancer activities. It also contains a high amount of ergosterol which can be converted to vitamin D2 whenexposing to UV light. Oyster mushroom powder was irradiated with UV-B for 180 min and extracted with 95% ethanol.Mushroom extract was determined for vitamin D2 concentration, total phenolic compound, antioxidative activity, tyrosinaseinhibitory property and cytotoxicity effect on human keratinocytes (HaCaT) and murine melanoma cells (B16F10) by MTTassay. The results demonstrated that the concentration of vitamin D2 of irradiated oyster mushroom extract was 153.96 ?g/g,which is 13 times higher than that of non-irradiated mushroom extract. Total phenolic content, antioxidative and tyrosinaseinhibitory activities of the two mushroom extracts were not significantly different. Neither oyster mushroom extract hada cytotoxic effect on keratinocytes, but on the other hand both inhibited the growth of murine melanoma cells. |
