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Evaluation of Palm PCRTM G1-12 System:a portable battery-operated PCR thermal cycler |
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| รหัสดีโอไอ | |
| Creator | 1. Siti Aminah Ahmed 2. Hong Leong Cheah 3. Nithya Ravichantar 4. Subash C.B. Gopinath 5. Thean Hock Tang |
| Title | Evaluation of Palm PCRTM G1-12 System:a portable battery-operated PCR thermal cycler |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2559 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 38 |
| Journal No. | 4 |
| Page no. | 343-348 |
| Keyword | hand-held PCR,convective PCR,portable thermal cycler |
| ISSN | 0125-3395 |
| Abstract | Polymerase chain reaction (PCR) is the basis of recombinant and other molecular biological techniques. Availability ofcheap and robust PCR platforms enables the tests to be performed easily, even in resource constrained settings. Hereinwe compared the efficacy of a portable thermal cycler ( Palm PCRTM G1-12 System) for rapid DNA amplification againstthe standard Peltier-based thermal cycler using plasmid DNA and genomic DNA in single and multiplex PCR experiments.Our study revealed that the Palm PCRTM G1-12 System could be a portable DNA amplification system to conduct variousmolecular techniques, especially in places where resources are limited. |
