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Levan-type fructooligosaccharide production using Bacillus licheniformisRN-01 levansucrase Y246S immobilized on chitosan beads |
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| รหัสดีโอไอ | |
| Creator | 1. Surawut Sangmanee 2. Santhana Nakapong 3. Rath Pichyangkura 4. Kamontip Kuttiyawong |
| Title | Levan-type fructooligosaccharide production using Bacillus licheniformisRN-01 levansucrase Y246S immobilized on chitosan beads |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2559 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 38 |
| Journal No. | 3 |
| Page no. | 295-303 |
| Keyword | LFOS,levansucrase,Bacillus licheniformis,immobilization,chitosan |
| ISSN | 0125-3404 |
| Abstract | Bacillus licheniformis RN-01 levansucrase Y246S (LsRN-Y246S) was immobilized by covalently linking onto chitosan,Sepabead EC-EP, and Sepabead EC-HFA, beads. The stability of immobilized LsRN-Y246S was found to be the highest withchitosan beads, retaining more than 70% activity after 13 weeks storage at 4 oC, and 68% activity after 12 hours incubation at40?C. LsRN-Y246S immobilized on chitosan beads withstands sucrose concentrations up to 70% (w/v), retaining over 85% ofits activity, significantly better than LsRN-Y246S immobilized on others supporting matrices. LsRN-Y246S immobilized onchitosan showed a 2.4 fold increase in activity in the presence of Mn2+, and gave slight protection against deactivation byof Cu2+, Zn2+, Fe3+, SDS and EDTA. A maximum of 8.36 g and an average of 7.35 g LFOS yield at least up to DP 11 can beproduced from 25 g of sucrose, during five production cycles. We have demonstrated that LFOS can be effectively producedby chitosan immobilized LsRN-Y246S and purified. |
