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Increase in production of biosurfactant from Oceanobacillus sp. BRI 10using low cost substrates |
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| รหัสดีโอไอ | |
| Creator | 1. Priyanka Parhi 2. VipraVijay Jadhav 3. Rama Bhadekar |
| Title | Increase in production of biosurfactant from Oceanobacillus sp. BRI 10using low cost substrates |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2559 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 38 |
| Journal No. | 2 |
| Page no. | 207-211 |
| Keyword | antarctic,biosurfactant,cost effective,glycolipoprotein,sugarcane juice,yield |
| ISSN | 0125-3395 |
| Abstract | The Antarctic isolate Oceanobacillus sp. BRI10 producing biosurfactant(BS) was cultivated in media containingdifferent low cost carbon and nitrogen sources. Initially glucose in basal salt medium was replaced individually with sugarcanejuice, whey and local commercial table sugar. Maximum emulsification index (E24) of 67.4% was obtained with sugarcanejuice. Further, effect of various nitrogen sources was examined on BS production. Among them sodium nitrate was found to bethe most suitable compound. E24 increased to 68.74% in the presence of sugarcane juice and sodium nitrate. The yield ofbiosurfactant in this medium was 14.25g l-1. The chemical characterization of biosurfactant revealed its glycolipoproteinnature consisting of lipids, carbohydrates, and proteins in the ratio of 4:94:2. Our results indicate a 14-fold increase in theyield and eight times decrease in the cost of production without major difference in the chemical nature of the biosurfactant,with respect to control. This is a significant result with regards to scale-up studies, recovery, and application. |
