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Suitable conditions for xylanases activities from Bacillus sp. GA2(1) andBacillus sp. GA1(6) and their properties for agricultural residues hydrolysis |
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รหัสดีโอไอ | |
Creator | 1. Sudathip Chantorn 2. Chanitchote Piyapittayanun 3. Phataraporn Khumphai 4. Srisuda Pannanusorn 5. Kulwadee Phannachet 6. Jirawan Apiraksakorn |
Title | Suitable conditions for xylanases activities from Bacillus sp. GA2(1) andBacillus sp. GA1(6) and their properties for agricultural residues hydrolysis |
Publisher | Research and Development Office, Prince of Songkla University |
Publication Year | 2559 |
Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
Journal Vol. | 38 |
Journal No. | 2 |
Page no. | 177-182 |
Keyword | Bacillus sp.,xylanase,mannanase,cellulase,agricultural wastes |
ISSN | 0125-3395 |
Abstract | Bacillus sp. GA2(1) and Bacillus sp. GA1(6) were isolated from soybean field in Khon Kaen province, Thailand. Crudeenzymes from both isolates showed the activities of cellulase, xylanase, and mannanase at 37?C for 24 h. The highest xylanaseactivities of Bacillus sp. GA2(1) and Bacillus sp. GA1(6) were 1.58?0.25 and 0.82?0.16 U/ml, respectively. The relativexylanase activities from both strains were more than 60% at pH 5.0 to 8.0. The optimum temperature of xylanases was 50?C inboth strains. The residual xylanase activities from both strains were more than 70% at 60?C for 60 min. Five agricultural wastes(AWs), namely coffee residue, soybean meal, potato peel, sugarcane bagasse, and corn cobs, were used as substrates forhydrolysis properties. The highest reducing sugar content of 101?1.32 ?g/ml was obtained from soybean meal hydrolysateproduced by Bacillus sp. GA2(1) xylanase. |