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Concentration of eicosapentaenoic acid (EPA) and docosahexaenoic acid(DHA) of Asian catfish oil by urea complexation: optimization of reaction conditions |
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รหัสดีโอไอ | |
Creator | 1. Pornpisanu Thammapat 2. Sirithon Siriamornpun 3. Patcharin Raviyan |
Title | Concentration of eicosapentaenoic acid (EPA) and docosahexaenoic acid(DHA) of Asian catfish oil by urea complexation: optimization of reaction conditions |
Publisher | Research and Development Office, Prince of Songkla University |
Publication Year | 2559 |
Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
Journal Vol. | 38 |
Journal No. | 2 |
Page no. | 163-170 |
Keyword | Asian catfish,omega-3 fatty acid,optimization,Pangasius bocourti,response surface |
ISSN | 0125-3395 |
Abstract | Optimization of the concentrating conditions of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) extractedfrom Asian catfish oil was studied to obtain a maximum concentration. The crude fish oil was extracted from the belly flapand adipose tissue of Asian catfish, and the extracted oil was used as fresh crude oil. The EPA and DHA were concentrated bythe urea complexation method. A hexagonal rotatable design was applied to examine the effects of crystallization temperatureand urea-to-fatty acid ratio on the total content of EPA and DHA (Y1) and the liquid recovery yield (Y2). The second orderpolynomial regression models for Y1 and Y2 were employed to generate the response surfaces. Under the optimum conditionsof -20 ?C and a urea-to-fatty acid ratio of 4 (w/w), the total concentration of EPA and DHA could be increased by up to 88%, whilea liquid recovery yield of 26% was obtained. |