รหัสดีโอไอ

รหัสดีโอไอ 10.14457/TU.the.2023.6
Title Penetration and antibacterial ability in dentinal tubules of neem paste for caries treatment (in vitro)
Creator Kamonchanok Tangsapsathit
Contributor Parinda Tasanarong, Advisor
Publisher Thammasat University
Publication Year 2023
Keyword Azadirachta indica, Neem, Antibacterial, L. rhamnosus, Penetration
Abstract Dental caries is the most common oral health issue worldwide. Untreated caries causes deterioration to the pulp and periapical tissues, eventually leading to tooth loss. Foreign organisms, like the bacteria Lactobacillus rhamnosus, can penetrate to dentinal tubules and develop under pulp liner material, resulting in caries progression and pulp inflammat ion. Azadirachta indica var. siamensis valeton (neem) leaf extract has been reported to possessantibacterial properties effective against oral acidogenic bacteria. Objectives: This study proposes to assess neem paste's antibacterial efficacy against L. rhamnosus in dentinal tubules.Methods: The bioactive constituents of neem extract were investigated using gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography(HPLC). The physicochemical properties of neem paste were investigated using visual, pH andviscosity measurements. The antimicrobial activity of neem pastes on L. rhamnousus, was alsoinvestigated, using an agar disc diffusion assay while the antimicrobial properties in dentinwere examined using LIVE/DEAD BacLight® staining and confirmed by confocal laser scanning microscope (CLSM). Results: Phytol was determined to be the primary constituent compound (65.97 percent). Others active compounds, including azadirachtin, querecetin, nimbin, and nimbidin weredetected. Neem paste penetrated the dentinal tubules by 1,030 ± 39.61 μm. Neem pasteinhibited L. rhamnosus growth in an agar disc diffusion assay and eliminated L. rhamnosus indentinal tubules at all experiment dentin depths.Conclusion: Neem paste has satisfactory antibacterial activity and highly effective penetrationthrough infected dentin. Therefore, neem paste might be developed to be an alternative materialfor indirect pulp treatment.
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บรรณานุกรม

Kamonchanok Tangsapsathit และผู้แต่งคนอื่นๆ. (2023) Penetration and antibacterial ability in dentinal tubules of neem paste for caries treatment (in vitro). Thammasat University:ม.ป.ท.
Kamonchanok Tangsapsathit และผู้แต่งคนอื่นๆ. 2023. Penetration and antibacterial ability in dentinal tubules of neem paste for caries treatment (in vitro). ม.ป.ท.:Thammasat University;
Kamonchanok Tangsapsathit และผู้แต่งคนอื่นๆ. Penetration and antibacterial ability in dentinal tubules of neem paste for caries treatment (in vitro). ม.ป.ท.:Thammasat University, 2023. Print.