| รหัสดีโอไอ | 10.14457/TU.the.2022.235 |
|---|---|
| Title | Transcriptome sequencing of eucalyptus responses to gall wasp infestation |
| Creator | Suparat Pinsupa |
| Contributor | Kittipat Ukotkij, Advisor |
| Publisher | Thammasat University |
| Publication Year | 2022 |
| Keyword | RNA sequencing, Transcriptome analysis, Eucalyptus, Leptocybe invasa, Eucalyptus gall wasp, Galling insect, TPS03, GCN5, ABCF5, RCA, SUI1 |
| Abstract | Leptocybe invasa is a gall wasp causing significant damage to Eucalyptus species. This study used RNA sequencing (RNA-seq) to identify differentially expressed genes (DEGs) associated with early L. invasa infestation in eucalyptus parents and their F1-progenies. A total of 14,648 significant DEGs were identified from U22-tolerant and C153-susceptible parents, and extremely tolerant and susceptible pools of their F1-progenies. A total of 324 out of 632 DEGs specific for the tolerant group were up-regulated, whereas 308 were down-regulated. A total of 182 out of 205 DEGs specific for the susceptible group were up-regulated, whereas 23 were down-regulated. Expression analysis by qRT-PCR of the selected DEGs was comparable with the results of RNA-seq. Expression analysis of the selected genes using top 5 progenies from each pool was consistent with that in the parents. Three genes were up-regulated after infestation in all tested tolerant plants, suggesting their important roles in the tolerant phenotype. These genes encoded rubisco activase; RCA, translation initiation factor SUI1 family protein; SUI1, and general control non-repressible 5; GCN5. Using expression and STRING analysis, our results suggesting that early response at three days after gall wasp infestation increased protein and terpenoid synthesis, and increased transportation of these molecules. In addition, wounding also increased photosynthesis and glycolysis. These processes involved interaction of several plant hormones, such as JA, Auxin, and ABA. The genes involved in these processes could play critical roles in the tolerant phenotype. The information obtained from this study could be useful for future Eucalyptus breeding programs. |
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Digital File #1 |