รหัสดีโอไอ 10.14457/TU.the.2019.1315
Title Effect of ethanolic extraction and partial purification on phenolic compound and antioxidant capacities of Caesalpinia sappan heartwood
Creator Aylada Kaenu
Contributor Supranee Kongkam, Advisor
Publisher Thammasat University
Publication Year 2019
Keyword Antioxidant activity ,Caesalpinia sappan L. ,MRC-5 cells ,Hydrogen peroxide
Abstract Caesalpinia sappan (CS) L. is a plant of Leguminosae family, commonly known as sappanwood, and Brazil or Fang (in Thai). It has red dry and was used as a coloring agent in beverage, food, garment, cosmetics, and traditional medicine. Brazilin is a major active compound naturally that found in the CS heartwood and also has indicated high antioxidant activities. In this study, the different ethanol concentrations and partial purification were used for the extraction of CS heartwood and the antioxidant capacity of the extracts was investigated in a chemical-based and cell-based assay. The CS heartwood was extracted with 95%, 75%, and 50% ethanol and partial purification using deionized water, dichloromethane, and ethyl acetate. The fractions were subjected to different antioxidant assays based on free radical scavenging activity by chemical-based assay and cell-based assay using H2O2-induced MRC-5 cells oxidative stress model. Additionally, the content of total phenolic and flavonoid was determined. The present study provides evidence that the total phenolic content and antioxidant capacity of CS heartwood extracts were significantly dependent on the ethanol concentration used for extraction. However, the subsequent ethyl acetate fraction showed the overall strongest anti-oxidative activity in the chemical-based assays. In the present study, the crude extract prepared by using 75% ethanol is the best of antioxidant properties. This extract contains a considerable amount of total phenolic (741.84 mg GAE/g CS heartwood extract), with low IC50 values (2.40 µg/mL in DPPH assay, 2.98 µg/mL in ABTS assay, and 14.21 µg/mL in lipid peroxidation assay), decrease enzyme activities (13.35, 11.83, and 10.71 U/mg protein in CAT assay, 2.21, 2.13, and 1.99 U/mg protein in SOD assay), and reduce intracellular ROS with MDA levels in MRC-5 cells (76.67%, 73.65%, and 63.42% in CAA assay, and 0.97 and 1.13 nmol MDA/mg protein in lipid peroxidation assay). Furthermore, total phenolic content and antioxidant capacity were closely correlated (p-value <0.01). Thus, these results suggest that 75% ethanol extract supports significant a good antioxidant potential natural of CS heartwood extract against oxidative stress and could be a candidate for the effectiveness of antioxidant supplements.
ดิจิตอลไฟล์ Digital File #1

บรรณานุกรม

Aylada Kaenu และผู้แต่งคนอื่นๆ. (2019) Effect of ethanolic extraction and partial purification on phenolic compound and antioxidant capacities of Caesalpinia sappan heartwood. Thammasat University:ม.ป.ท.
Aylada Kaenu และผู้แต่งคนอื่นๆ. 2019. Effect of ethanolic extraction and partial purification on phenolic compound and antioxidant capacities of Caesalpinia sappan heartwood. ม.ป.ท.:Thammasat University;
Aylada Kaenu และผู้แต่งคนอื่นๆ. Effect of ethanolic extraction and partial purification on phenolic compound and antioxidant capacities of Caesalpinia sappan heartwood. ม.ป.ท.:Thammasat University, 2019. Print.