รหัสดีโอไอ 10.14457/CU.the.2009.1682
Title Synthesis and protein immobilization of poly(acrylic acid) brushes for stem cell culture
Creator Sasithon Rodtamai
Contributor 1. Voravee P. Hoven
2. Nipan Israsena Na Ayudhaya
Publisher Chulalongkorn University
Publication Year 2009
Keyword Poly(acrylic acid) ,การตรึงโปรตีน
Abstract Surface-tethered poly(acrylic acid) (PAA) brushes were prepared by surface initiated polymerization of tert-butyl acrylate from glass substrates via activator regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP) followed by hydrolysis using methanesulfonic acid. Upon using an optimized condition, poly(tert-butyl acrylate) brushes having a graft density of approximately 0.33 chains/nm² were obtained. Carboxyl groupdensityof the PAA brushes can be varied as a function of the chain length or molecular weight ranging from 0.7 x10⁻⁹ to 1.6 x10⁻⁹ mol/cm². It has been demonstrated that the carboxyl groups of PAA brushes are readily available for attachment of BSA and N-cadherin mimic cyclic peptide. The successive surface modification via polymer brushes formation and protein/peptide immobilization were also verified by ATR-FTIR analysis and water contact angle measurements. According to cytotoxicity test against mouse neural progenitor cell, it was found that the immobilized N-cadherin mimic cyclic peptide on PAA brushes was non toxic and provided a level of cell compatibility equivalent to that of the surface coated with laminin, a commonly used adhesive protein for stem cell culture suggesting its potential in stem cell culture applications.
URL Website cuir.car.chula.ac.th
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บรรณานุกรม

Sasithon Rodtamai และผู้แต่งคนอื่นๆ. (2009) Synthesis and protein immobilization of poly(acrylic acid) brushes for stem cell culture. Chulalongkorn University:ม.ป.ท.
Sasithon Rodtamai และผู้แต่งคนอื่นๆ. 2009. Synthesis and protein immobilization of poly(acrylic acid) brushes for stem cell culture. ม.ป.ท.:Chulalongkorn University;
Sasithon Rodtamai และผู้แต่งคนอื่นๆ. Synthesis and protein immobilization of poly(acrylic acid) brushes for stem cell culture. ม.ป.ท.:Chulalongkorn University, 2009. Print.