|
HPLC-QTOF-MS method for quantitative determinationof active compounds in an anti-cellulite herbal compress |
|---|---|
| รหัสดีโอไอ | |
| Creator | 1. Ngamrayu Ngamdokmai 2. Neti Waranuch 3. Krongkarn Chootip 4. Nitra Neungchamnong 5. Kornkanok Ingkaninan |
| Title | HPLC-QTOF-MS method for quantitative determinationof active compounds in an anti-cellulite herbal compress |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2017 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 39 |
| Journal No. | 4 |
| Page no. | 463 |
| Keyword | HPLC-QTOF-MS,herbal compress,quality control,traditional Thai medicines |
| ISSN | 0125-3395 |
| Abstract | A herbal compress used in Thai massage has been modified for use in cellulite treatment. Its main active ingredientswere ginger, black pepper, java long pepper, tea and coffee. The objective of this study was to develop and validate an HPLCQTOF-MSmethod for determining its active compounds, i.e., caffeine, 6-gingerol, and piperine in raw materials as well as inthe formulation together with the flavouring agent, camphor. The four compounds were chromatographically separated.The analytical method was validated through selectivity, intra-, inter day precision, accuracy and matrix effect. The resultsshowed that the herbal compress contained caffeine (2.16 mg/g), camphor (106.15 mg/g), 6-gingerol (0.76 mg/g), and piperine(4.19 mg/g). The chemical stability study revealed that herbal compresses retained >80% of their active compounds after1 month of storage at ambient conditions. Our method can be used for quality control of the herbal compress and its rawmaterials. |
