รหัสดีโอไอ 10.14455/TSB.res.2019.26
Title Exploration of cassava lincRNAs relevant to defense response against cassava brown streak virus (CBSV) infection
Creator Saowalak Kalapanulak
Contributor 1. Rungaroon Suksamran
2. Treenut Saithong
3. Chinae Thammarongtham
Publisher The Thai Society for Biotechnology
Publication Year 2019
Keyword CBSV ,Disease Resistance Gene ,LincRNA ,Transcriptome
Abstract Cassava brown streak virus (CBSV) poses a huge threat to cassava production and can lead to substantial yield and quality losses. Understanding of plant gene regulation against CBSV infection is necessary for developing resistant cultivars. Transcriptome analysis offers insights into the underlying gene regulation and defense mechanisms against pathogens. However, most of the CBSV-related transcriptome analysis in cassava has mainly focused on protein coding genes, and comprehensive knowledge of the roles of long noncoding RNAs (lncRNAs), which have been linked to the defense mechanisms of some plants, remains elusive for cassava. Here, we performed a transcriptome- wide screening for long intergenic non- coding RNAs (lincRNAs) in a CBSV-resistant cultivar under CBSV infection. A total of 415 expressed lincRNAs were found to be distributed across the cassava genome. Twenty-nine of them were significantly differentially expressed relative to control (absolute log2 fold change ? 2 and corrected p-value < 0.05). Among these, 4 were potentially involved in regulating transcripts of disease resistance proteins, heat shock proteins, chaperones, late embryogenesis abundant proteins, major latex proteins, stress-responsive GRAS transcription factors, and hormone-regulated proteins at the early stage of infection. To our knowledge, this work provides the first evidence of lincRNAs involved in cassava defense response against CBSV. It provides insightful information towards a comprehensive understanding of post-transcriptional regulation in plants against viral infection, for which further wet lab experimentation is crucial.
Language EN
URL Website http://tsb2019.com/
Website title The 31st Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2019)
ดิจิตอลไฟล์ Digital File

บรรณานุกรม

Saowalak Kalapanulak และผู้แต่งคนอื่นๆ. (2019) Exploration of cassava lincRNAs relevant to defense response against cassava brown streak virus (CBSV) infection. The Thai Society for Biotechnology:ม.ป.ท.
Saowalak Kalapanulak และผู้แต่งคนอื่นๆ. 2019. Exploration of cassava lincRNAs relevant to defense response against cassava brown streak virus (CBSV) infection. ม.ป.ท.:The Thai Society for Biotechnology;
Saowalak Kalapanulak และผู้แต่งคนอื่นๆ. Exploration of cassava lincRNAs relevant to defense response against cassava brown streak virus (CBSV) infection. ม.ป.ท.:The Thai Society for Biotechnology, 2019. Print.