รหัสดีโอไอ 10.14456/ea.2019.2
Creator 1. Wanida Petlamul
2. Sawai Boukaew
Title Optimisation and Stabilisation of Cellulase and Xylanase Production by Beauveria bassiana
Contributor -
Publisher Thai Society of Higher Education Institutes on Environment
Publication Year 2019
Journal Title EnvironmentAsia
Journal Vol. 12
Journal No. 1
Page no. 10 -19
Keyword Beauveria bassiana ,Cellulase ,Optimisation ,Stabilty ,Xylanase
URL Website http://www.tshe.org/ea/index.html
Website title EnvironmentAsia
ISSN 1906-1714
Abstract This research was conducted to optimise and stabilise cellulase and xylanase production by Beauveria bassiana B14532. Optimisation for pH (4.0, 6.5 and 9.0) and temperature (30, 55 and 80 degree C) was attained by a response surface methodology (RSM) model to fit the data using central composite design (CCD). Results indicated that B. bassiana B14532 recorded cellulase and xylanase activity at 3.59 U/mL (144h) and 39 U/mL (120h) respectively. Optimum pH and temperature which produced the highest cellulase (3.89 U/mL) were 6.5 and 80 degree C respectively while highest xylanase (41.01 U/mL) was obtained from optimum pH 6.5 and temperature 55 degree C. Coefficients of determination (r-square) were 0.93 (cellulase activities) and 0.98 (xylanase activities). Stability of the cellulase and xylanase enzymes was investigated at the optimised pH values and temperatures for 120 min. Results showed that both enzymes decreased as reaction time increased by every 10 min. Cellulase retained 88, 80, 74, 66 and 55% of its activity at pH 6.5, 80 degree C post incubation for 50 min, while xylanase retained higher enzyme values at 93, 91, 83, 70, 65, 61 and 50% of its activity at pH 6.5, 55 degree C post incubation for 70 min.
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บรรณานุกรม

Wanida Petlamul และผู้แต่งคนอื่นๆ. (2019) Optimisation and Stabilisation of Cellulase and Xylanase Production by Beauveria bassiana. EnvironmentAsia,12,10 -19.
Wanida Petlamul และผู้แต่งคนอื่นๆ. "Optimisation and Stabilisation of Cellulase and Xylanase Production by Beauveria bassiana". EnvironmentAsia 12 (2019):10 -19.
Wanida Petlamul และผู้แต่งคนอื่นๆ. Optimisation and Stabilisation of Cellulase and Xylanase Production by Beauveria bassiana. Thai Society of Higher Education Institutes on Environment:ม.ป.ท. 2019.